Purification of acidic protease from the cotyledons of germinating Indian bean (Dolichos lablab L. var lignosus) seeds
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چکیده
منابع مشابه
Mobilization of albumins and globulins during germination of Indian bean (Dolichos lablab L. var. lignosus) seeds
Albumin and globulin fractions of Indian bean seeds exhibit different mobilization patterns during the 8-day germination period. The albumin degradation was lesser in the early stage and reached a maximum in the later stages of germination. However, the globulins were degraded steadily from the start of imbibition. The influence of the embryonic axis on albumin and globulin degradation was also...
متن کاملAxial control of protein reserve mobilization during germination of indian bean (Dolichos lablab L.) seeds
The influence of embryonic axis and exogenously applied plant growth hormones on protein mobilization and development of proteases have been investigated in Indian bean (Dolichos lablab L. var lignosus) seeds during germination and post-germinative growth up to 10 days. Accumulation of free amino acids synchronized with rapid proteolysis and higher levels were maintained throughout the germinat...
متن کاملProteases of germinating winged-bean (Psophocarpus tetragonolobus) seeds: purification and characterization of an acidic protease.
Two major classes of protease are shown to occur in germinating winged-bean (Psophocarpus tetragonolobus) seeds, by assaying extracts at pH 8.0 and pH 5.1 with [14C]gelatin as substrate. At pH 8.0, the activity profile of the enzyme shows a steady rise throughout the period of germination, whereas the activity at the acidic pH is very low up to day 5 and then increases sharply reaching a peak o...
متن کاملKaryotype analysis in lignosus bean (Dipogon lignosus) and lablab bean (Lablab purpureus)
The experiment was conducted in the cytogenetics laboratory of the Department of Genetics and Plant Breeding of Bangladesh Agricultural University during July 2008 to September 2009 aiming to analyze and compare the karyotypes of two genotypes of Lignosus bean (Dipogon lignosus L.) and Lablab bean (Lablab purpureus L.). Root tips from germinated seeds were collected, pretreated and fixed in ace...
متن کاملMitochondrial Arginase Activity from Cotyledons of Developing and Germinating Seeds of Vicia faba L.
Differential and sucrose density gradient centrifugation established that about 80% of the total arginase activity (EC 3.5.3.1) in cotyledons of germinating broad bean seeds (Vicia faba L.) was present in the mitochondrial fraction. The mitochondrial arginase activity was enhanced considerably by exposure to osmotic shock, by freezing and thawing, or by Triton X-100 treatment. About 10% of the ...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2005
ISSN: 1684-5315
DOI: 10.5897/ajb2005.000-3130